We are seeking a highly organized and detail-oriented Cell Culture Technician to manage daily operations and maintain the integrity of our Biosafety Level 2 (BSL-2) cell culture laboratory. The ideal candidate will have robust hands-on experience with mammalian cell lines and primary cell cultures, good aseptic technique, and a strong commitment to enforcing biosafety compliance. You will ensure the lab remains audit-ready, equipment is operational, and research teams are supported with high-quality, contamination-free cell lines.

Essential Duties include:

• Cell Line Maintenance: Routinely passage, feed, bank, and thaw mammalian cell lines (including primary cells or human-derived lines). Monitor cell growth, confluence, and morphology daily. Provide other lab members cells and medium.
• BSL-2 Safety & Compliance: Enforce laboratory safety protocols in accordance with institutional and federal guidelines. Ensure proper usage of Personal Protective Equipment (PPE)
• Waste Management: Safely treat and dispose of liquid and solid biohazardous waste according to standard protocols (e.g., chemical bleaching, autoclaving, and sharps disposal).
• Equipment Upkeep: Perform routine maintenance, deep cleaning, and calibration tracking for Class II Biosafety Cabinets (BSCs), CO₂ incubators, centrifuges, and water baths. Coordinate annual professional certifications.
• Quality Control: Conduct routine testing for mycoplasma, sterility, and cross-contamination. Keep flawless records of cell line passages, batch histories, and repository logs.
• Inventory & Media Prep: Prepare standard cell culture media, buffers, and reagents under sterile conditions. Manage laboratory inventory, tracking stock levels of plastics, PPE, and reagents.
• Ability to comply with personal protective equipment requirements.

Minimum Qualifications:

• Typically requires a minimum of 2-4 years of relevant cell handling experience.
• Proven mastery of aseptic technique.
• Experience working within a Biosafety Level 2 (BSL-2) environment.
• Proficiency with inverted microscopes, hemocytometers/automated cell counters, and cryostorage systems.
• Strong organizational habits and documentation practices, and the ability to work independently in a fast-paced environment.

Preferred Qualifications:

• Associate or Bachelor’s degree.
• Hands-on experience in a dedicated cell culture environment (academic or industry).

The estimated pay range for this position is $30 - $35/hour. Within the range, individual pay is determined by work location and additional factors, including job-related skills, experience, and relevant education or training.